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½Ç½Ã°£ ÇÙ»ê ºÐ¼®±â [ Real Time Polymer Chain Reaction System(RT-PCR) ]
¤±¸ðµ¨/Á¦ÀÛ»ç : Applied Biosystems 7500 Real-time PCR System / Applied Biosystems / USA
¤±±â±â´ã´ç : ¹ÚÁø¿µ
¤±ÀüȹøÈ£ : 061 - 450 - 6138
¤±ÀüÀÚ¿ìÆí : 28totoro@hanmail.net
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Çü±¤Ç¥Áö ¹°Áú, 5 ' Nuclease assay(TaqMan Probe), Double-stranded DNA binding dye, SYBR Green IÀ» ÀÀ¿ëÇÏ¿© sample³»¿¡ Á¸ÀçÇÏ´Â target DNA/RNAÀÇ ¾çÀ» ½Ç½Ã°£À¸·Î ÃøÁ¤ (Real-time DNA/RNA Quantitation)ÇÏ´Â ÀåºñÀÌ´Ù. ÀÌ Àåºñ´Â tungsten Halogen lamp¿¡¼ ³ª¿À´Â ºûÀ» °¢°¢ÀÇ well¿¡ 96°³ÀÇ tube·Î ºÐ»ç½ÃÄÑ À̷κÎÅÍ ¹æÃâµÈ Çü±¤À» °¨µµ°¡ ¶Ù¾î³ CCD camera·Î °ËÃâÇÑ´Ù. ÀÌ ¹æ½ÄÀ¸·Î background¸¦ ³·Ãâ ¼ö ÀÖÀ¸¸ç, °¢ well¸¶´Ù detector°¡ ¿¬°áµÇ¾î ÀÖ¾î ´ÜÀ§½Ã°£¿¡ ´õ ¸¹Àº data¸¦ ¾òÀ» ¼ö ÀÖ¾î °¨µµ°¡ ¶Ù¾î³ª¸ç º¸´Ù Á¤È®Çϰí ÀçÇö¼ºÀÖ´Â °á°ú¸¦ ¾òÀ» ¼ö ÀÖ´Ù
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- 5 excitation filters, 5 emission filters: FAM¡¡/SYBR Green I, VIC/JOE, NED/TAMRA/CY3 Dye, ROX/Texas Red, CY5 Dye¡¡¿¡ optimization µÇµµ·Ï °í¾ÈµÇ¾î ÀÖÀ½
- Excitation sourceÀÎ tungsten-halogen lampÀÇ life time°ú current status¸¦ monitoring
- 9 logs of linear dynamic range
- Small instrument footprint
- Chemistry: TaqMan Chemistry, SYBR Green Chemistry ¹× ±× ¿ÜÀÇ ¸ðµç methods
- Application: Gene expression analysis, Pathogen quantitation, SNP genotyping, Plus/minus assay
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3. ¾Ï À¯¹ß À¯ÀüÀÚÀÇ °Ë»ö ¹× ´ë»ç¿Í °ü·ÃÇÑ À¯ÀüÀÚ Typing, Allele detection°ú Single Nucleotide Polymorphism (SNP)µîÀ» ÀÌ¿ëÇÑ mutation °ËÃâ
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